METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF OLMESARTAN, CLINIDIPINE & CHLORTHALIDONE BY RP-HPLC METHOD

Muttuju Navya Sri*, Dr. Devanaboyina Narendra and Gadi Vijaya Lakshmi

ABSTRACT

A simple, Accurate, precise method was developed for the simultaneous estimation of the Olmesartan, Cilinidipine and Chlorthalidone in liquid dosage form. Chromatogram was run through Ascentis C18 150x4.6mm,5. Mobile phase containing Buffer and Acetonitrie in the ratio of 43:57 and was pumped through column at a flow rate of 0.8ml/min. Buffer used in this method was 0.01N NH4. Temperature was maintained at 30°C. Optimized wavelength for Olmesartan. Cilinidipine and Chlorthalidone was 230nm. Retention time of Olmesartan, Cilinidipine and Chlorthalidone. Were found to be 2.253 min, 2.701min and 3.598 min. %RSD of method precision for Olmesartan, Cilinidipine and Chlorthalidone were and found to be 0.5, 0.2 and 0.6 respectively. % recovery was Obtained as 100.15%, 100.09% and 100.01% for Olmesartan, Cilinidipine and Chlorthalidone. respectively. LOD values are obtained from regression equations of Olmesartan, Cilinidipine and Chlorthalidone. were 0.24ppm, 0.01ppm, 0.01ppm, LOQ values are 0.72ppm, 0.02ppm and 0.04 ppm respectively. Regression equation of Olmesartan was y = 23139.x + 15862, Cilinidipinee was y = 6442x +1907 and of Chlorthalidone was y = 8372.x + 3094. Retention times are decreased so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

Keywords: Olmesartan, Cilinidipine, Chlorthalidone, RP-HPLC.