MICROBIAL PROTEASE –SCREENING CONFIRMATION AND BIOSYNTHESIS THROUGH SUBMERGED FERMENTATION FROM ASPERGILLUS SP

Pavan Kumar K., Siddalingeshwara K. G., Prakruthi G. and Sumana K.*

ABSTRACT

Proteases occur every living being in nature. They are secreted inside or outside of the cells for various physiological functions. Proteases have been successfully produced by researchers from different microbial sources. Microbes account a two-thirds share of commercial protease around the globe. Twelve Aspergillus sp were isolated from stressed soil samples from different areas in Bangalore. Isolated fungal strains were screened for Protease production by plate assay (casein plate). Aspergillus sp KP 10were selected as best protease producer (1.08 cm) and were confirmation carried out by Thin layer chromatography (Rf value is 0.71). The Aspergillus sp KP 10 were employed for further biosynthesis of protease studies. The different pH (6), temperature (300C) and inoculum size (1.25 ml) were optimized and it showed 1.9 IU of protease activity.

Keywords: Protease, thin layer chromatography, Plate assay and Aspergiluus sp.