OPTIMIZATION OF EXTRACELLULAR PROTEASE EXTRACTED FROM ESCHERICHIA COLI

Basima Khalid Abed*, Sawsan Hassan Authman* and Khadim Hashim Yassein*

ABSTRACT

Protease production from Escherichia coli isolated from patient suffering Urinary Tract Infection (UTI) was studied. The ability of this bacteria to produce protease enzyme was screening on skim milk agar , as well as the culture conditions like culture medium , inoculum concentration , pH , temperature , incubation period were optimized. The high level of protease production of isolated bacteria (E.coli 1 , E.coli 2) were found in Tryptic soy broth culture medium with inoculum concentration 1.5×108 CFU/ml at 40 °C after 24 hrs at pH = 6 with continuous agitation (150 rpm).

Keywords: Protease, E.coli, UTI, Optimization.