IN VTTRO ANTI SICKLING EFFECT OF ARTEMISIA HERBA ALBA ASSO EXTRACT ON SICKLE CELLS

Nusiba Abdullah Yousif, Toga Abdalazim Fadlalla, Rayan Hamid Omer, Mohammed Mobarak Elbasheir*, Wala Eldin Osman Elradi and Elharam Ibrahim Abdallah

ABSTRACT

Background: Sickle cell disease (SCD) is one of the major haemoglobinopathies, in which the abnormal haemogblobin altered the cell to sickle shape under low antioxidant status. The patients have increase in the free radicals which damage the cells. It has shown that some medicinal plants which contain antioxidant molecules as phenolics and flavonoids compounds have an anti-sickling activity, which indicates a new therapeutic way to combat and manage this disease. Objective: The current study aimed to determine the in vitro anti-sickling effect of Artemisia herba-alba asso (Chih) extracts. Materials and methods: Ten blood samples were taken from patients known to have Sickle cell disease (HB -SS) attending the Sickle Cell Clinic in Khartoum state used in the evaluation of the anti-sickling effect of the plant extracts in this study (each sample was tested 6 times with different concentrations (250, 500, and 1000 μg/ml) of aqueous and methanolic extracts of the plant and one time with normal saline as a control)). All samples were collected in EDTA container. Emmel test was used to assess anti-sickling activity of this plant. Then data were analyzed by SPSS. Results: A high significant increase in the percentage of unsickled Red blood cells with p-value (0.000) was observed in the presence of 1000, 500 and 250μg/ml of Artemisia herba-alba asso of both aqueous and methanolic extract. Conclusion: The result obtained in this study suggests the using of Artemisia herba Alba asso in the management of sickle cell disease complication as well as it’s a natural cost effective way for combating the disease. Further in vivo and in vitro studies are recommended to confirm this result.

Keywords: In vitro, Anti-sickling effect, Artemisia herba Alba asso, Unsickled red blood cells, Sickle cell disease.