EVALUATION OF ANTIMICROBIAL ACTIVITY OF LEAF EXTRACT OF TINOSPORA CORDIFOLIA ON URINARY TRACT INFECTION (UTI) CAUSING PATHOGENIC BACTERIA

Madan Mohan Gunda* and Rani Kommidi

ABSTRACT

Urinary tract infection (UTI) has become a more grievous problem today, due to multidrug resistance of infecting Gram-positive (GP) and Gram-negative (GN) bacteria, sometimes even with multiple infections. This study examines effectivity of Tinospora cordifolia Tropical flowering plant. T. cordifolia or “Guduchi” is a well-known medicinal plant studied extensively since ancient times. This article specifically describes the role of T. cordifolia as an antimicrobial agent and as an immunity enhancer plant. Pathogenic bacteria were isolated from urine samples of 70 school going children and were sent to AMPATH for Urine culture Antibiograms of 4 isolated bacteria (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia and Enterococcus faecalis ).were isolated from urine samples were ascertained by the disc-diffusion method, and antibacterial effectivity of plant extracts was monitored by the agar-well diffusion method. Isolated bacteria were floridly MDR (Multi Drug Resistant Bacteria) to most antibiotics of the day. Methanol extracts of T. cordifolia plant were used, and extracts at least caused 25–29 mm as the maximum size of zone of inhibition on bacterial lawns. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of methanol extracts of T. cordifolia plant were recorded. The Ethanol extract of P. aeruginosa, had 4.08 mg/ml as the lowest MIC value and 8.37 mg/ml as the lowest MBC value, against signifying effectivity; but it had the highest MIC value of 9.68 mg/ml. and the highest MBC value of 24.07 mg/ml for most other MDR bacteria including Escherichia coli. Qualitative phytochemical analysis was done for these T. cordifolia plant, which can be promoted as complementary medicine. In the present study, we examined the anti-microbial effects of dried and powered leaves of T. cordifolia. were extracted with Ethanol, Ethyl acetate, and hexane Solvents were used, process followed by soxhelt extraction.

Keywords: Medicinal plant; Tinospora cordifolia; solvent extracts; anti-oxidant activity and, Ethanol extract.