BIOFILM FORMING PROPERTY & ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF UROPATHOGENS CAUSING CATHETER ASSOCIATED URINARY TRACT INFECTION (CAUTI)

Kyaw Zeyar Soe* and Sanjay Pratap Singh

ABSTRACT

Introduction: Catheter Associated Urinary Tract Infection (CAUTI), a common healthcare-associated infection, is difficult to manage for biofilm producing microorganisms and this property is also predisposing by nature of devise material. Moreover, multi-drugs resistance uropathogens are one of the challenging problem in healthcare facility.
Aims and Objectives: Evaluation of biofilm forming properties and antimicrobial susceptibility of uropathogenic microorganisms isolated from cases of CAUTI in Latex rubber Foley’s catheters and Silicone-coated Foley’s catheter.
Materials and Methods: Aseptically collected Urine samples of 116 individuals qualified as CAUTI were investigated to obtain uropathogenic bacteria. Their biofilm forming properties & antimicrobial resistance patterns were also evaluated. The different biofilm forming properties of uropathogens in Latex rubber Foley’s catheter and Silicone-coated rubber Foley’s catheter was also analysed.
Results: During study period, 116 patients were labelled as CAUTIs and total CAUTI rate per thousand urinary catheter days was 5.7. A total of 116 bacteria were isolated from the CAUTI patients with Escherichia coli being the most frequent isolate 35%, followed by Klebsiella pneumonia, Pseudomonas aeruginosa, Enterococcus faecalis and other Gram negative organisms. Out of 116 isolates, 56% of bacteria were found to produce biofilms. Of these, 43% were strong biofilm producers and 13% observed as moderate biofilm producers. Majority of uropathogens were found to be resistance to most of the antibiotics had been tested.
Conclusion: Enterobacteriaceae were the most common isolates and the biofilm producing isolates were observed more resistant to antimicrobials agents. Most of the Gram negative isolates were multi-drugs resistant organisms (MDRO). Using Silicone-coated Foley’s catheter can not only delay the onset of Catheter Associated Urinary Tract Infection but also have lesser proportion of biofilm producing bacteria with lower chance of multi-drugs resistant organisms (MDRO) thereby ensuring better curative treatment for the patient.

Keywords: CAUTI, Biofilm, Uroparhogens, Antimicrobial resistance.