PHYTOCHEMICAL AND IN-VITRO PHARMACOLOGICAL EVALUATION OF IPOMOEA SEPIARIA AGAINST SELECTED PATHOGENIC MICROORGANISMS.

B. Prasanna Kumar*, K. Sravanthi, Pavan Kumar Chadalawada, B. Pooja, K. Vadivel

ABSTRACT

Background: Convolvulaceae - The Morning Glory Family, the genus Ipomoea since time immemorial have been in continuous use for different purposes, such as nutritional and somany importand medicinal values. There is no much more work was carried out on this species. Objective: To Study the preliminary phyto chemical parameters and in-vitro antimicrobial and anthelmintic acitvity on selected medicinal plant crude extract. Materials and Methods: In the present study antimicrobial activity of petroleum ether and ethyl acetate extracts from the leaves of Ipomoea sepiaria and phytochemicals was evaluated with Gram-positive and Gram-negative bacteria. The fungi employed for screening were Aspergillus niger and Candida albicans. Leaves were dried, powdered and extracted with soxhlet apparatus. In-vitro antibacterial and antifungal activity was performed by the agar diffusion method at concentrations of 50mg/mL to 150mg/mL. Ciprofloxacin (10 μg/mL) and Fluconazole (10μg/mL) was employed as antibacterial and antifungal reference standard to compare the results. Anti-helminthic activity also performed by using Pheretima posthuma. paralysis and death time was monitored. Albendazole was used as standard. Results: It has been observed that all the tested extracts showed mild to moderate activity against the bacteria and fungi whereas higher concentration of petroleum ether extract and ethyl acetate extract 150mg/mL were found to be most promising antibacterial activity and shows highest activity towards gram positive organisms. Plant extract shown comparable anthelmintic activity to that of albendazole as standard. Conclusion: We concluded that the petroleum ether and ethyl acetate extracts of Ipomoea sepiaria leafhave mild activity against bacteria and fungus but it revealed significant anthelmintic activity.

Keywords: Albendazole, Ciprofloxacin, Fluconazole, Ipomoea sepiaria, Pheretima posthuma.