PHYTO-PHARMACOLOGICAL AND IN-VITRO ANTICANCER ACTIVITY OF WEDELIA CHINESIS AGAINST HT-29 (HUMAN COLON CANCER)

Nithin Manohar R.*, Padmaja V., R. Rajkumar, Shiji Kumar P. S. and Ancy P.

ABSTRACT

In this study, we evaluated anticancer activity of ethanol extract of Wedelia chinesis (W.chinesis) against HT-29 (Human colon cancer) Cytotoxicity of W.chinesis was investigated using MTT assay. Doxorubicin was used as the positive control. MTT assay showed activity against the tested cell line. The cytotoxicity activities were expressed as percentage of cell viability. Cytotoxicity of ethanolic extract of W.chinesis against HT-29 cell line was measured and the IC50 value of ethanol extract of W.chinesis was 57.28±0.152 μg/mL μg/ml. IC50 value of Doxorubicin (standard) was 11.30±0.20μg/mL. Morphological alteration of HT-29 cells lines upon exposure using W.chinesis extract was observed under phase contrast microscope. The cells indicated the most prominent effects after exposure to the W.chinesis extract. The present findings confirmed cytotoxic effect of ethanol extract of W.chinesis HT-29 (Human colon cancer)

Keywords: anticancer, cell lines, Medicinal plants, herbs, cytotoxicity, flavanoids, polyphenolic compounds.