FERMENTATIVE PRODUCTION OF L-GLUTAMATIC ACID BY THE BACILLUS SPECIES ISOLATED FROM SEWAGE CONTAMINATED SOIL

Uppara Keerthi, Mokula Md. Rafi, Budolla Gowramma, Merza A Razak, C.V. Krishnarjun Reddy, A.V.N. Swamy and D. Muralidhara Rao*

ABSTRACT

Experiments were conducted to isolate glutamic acid producing bacteria from soils contaminated with sewage water. The bacteria were isolated from sewage contaminated soils in Anantapur city and cultured on agar plates and were screened for glutamic acid production by performing bioautographic technique. The glutamic acid producing strains were designated as INSPDST8, INSPDST9, SKUDBT03 and they were identified as new variants of B. flexus, B. stratosphericus, B. thuringenesis respectively by physiological, biochemical characteristics and with 16s rDNA sequencing. All the strains were gram positive, spore forming, and motile, aerobic, alkaliphilic bacteria. The optimum growth pH range was 8-10 and temperature 37 oC. The amount of glutamic acid produced by B. flexus, B. stratosphericus, B. thuringenesis was 76, 72, 78mg/100ml.

Keywords: Bacillus sps, glutamic acid, sewage soil, alkaliphilic bacteria.