FREE RADICAL SCAVENGING PROPERTY OF ?-AESCIN AND TRANS-CHALCONE: IN VITRO STUDY

Harsimran Singh*, Shabir Sidhu and MU Khan

ABSTRACT

The aim of the present study was to investigate the antioxidant activity of β-aescin and trans-Chalcone by using in-vitro DPPH assay. Ascorbic acid was used as standard antioxidant compound. β-aescin is active phytoconstituent found in the extract of horse chestnut (Aesculus hippocastanum L.) seed. trans-Chalcone is open chain flavonoid biosynthesized in plant, Piper methysticum and synthesized in laboratory by Claisen-Schmidt condensation. The concentration range of 10, 20, 40, 60, 80 and 100 μg/mL of both the drug solutions was used. Our data showed that β-aescin and trans-Chalcone exhibited antioxidant activity in a concentration dependent manner, indicated by % inhibition and IC50 value. The IC50 value of β-aescin, trans-Chalcone and ascorbic acid (standard drug) were found to be 1.54, 1.45 and 1.47 respectively. Moreover, on the basis of IC50 values, trans-Chalcone exhibited more potent antioxidant activity when compared with standard compound and β-aescin. The range of antioxidant behavior is trans-Chalcone > ascorbic acid > β-aescin.

Keywords: trans-Chalcone; ?-aescin; Oxidative stress.