SCREENING OF PHYTOCHEMICALS AND DETERMINATION OF ANTIHYPERGLYCAEMIC ACTIVITY ON ETHANOLIC EXTRACT OF FRESH FRUIT OF NEPHELIUM LAPPACEUM L. ON FEMALE SPRAGUE – DAWLEY RATS

Loo Jian Zhang, Nabila Perveen, Parasuraman S. and Naeem Hasan Khan*

ABSTRACT

Screening of phytochemicals and determination of antihyperglycaemic activity of ethanolic extracts of Nephelium lappaceum L (EENL) fresh fruit on female Sprague-Dawley rats. The extraction for Nephelium lappaceum was done by using 96% ethanol solvent and its pharmacological action was screened. Adult female healthy Sprague Dawley (SD) rats were used for the testing determination of the antidiabetic activity of EENL. In the screening, the rats were divided into 3 different groups with 3 animals each and tagged with standard control, 1mL and 2mL PO of 4000mg/kg EENL. Apart from the standard control group, the rest of the SD rats were given with a freshly prepare single dose intraperitoneal infection of 55mg/kg streptozotocin (STZ) to induce diabetes mellitus. Follow on the rats with diabetes mellitus was given with EENL for consecutives 21 days through oral route feeding. The blood sample was collected on 7th, 14th and 21st day via caudal vein. From the study, EENL shows it has the capabilities to reduce glucose level in the rats. However the different dosing for the rats did not show a significant change in the final result.

Keywords: Diabetes mellitus, Streptozotocin, Nephelium lappaceum, antihyperglycaemic.