A REVIEW ON UV SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS MULTICOMPONENT ANALYSIS

Amira H. Kamal*, Samah F. El-Malla and Sherin F. Hammad

ABSTRACT

In present era, market is floated with various combinations dosage forms and the number is increased day by day. These multicomponent formulations are gaining interest due to greater patient acceptability, increased potency, multiple action, fewer side effects, and quicker relief. Therefore, it is desired that these formulations meet the entire standards related to their quality, safety, and efficacy. This can only be possible if different analytical techniques are available for their determination. Different UV spectrophotometric methods are used in simultaneous multicomponent analysis. Such methods are based on recording and mathematically processing absorption spectra. This review is mainly focused on simultaneous equation method, difference spectrophotometry, derivative spectrophotometry, absorbance ratio spectra, derivative ratio spectra, double divisor ratio spectra derivative method, successive ratio - derivative spectra, Q-absorbance ratio method, isosbestic point method, absorpitivity factor method, dual wavelength method, ratio subtraction method, mean centering of the ratio spectra, absorption factor method and multivariate methods. An overview of theories and some applications of these methods are presented.

Keywords: spectrophotometric methods, multicomponent analysis, double divisor, successive ratio-derivative, dual wavelength, ratio subtraction, multivariate methods.