BIOREMEDIAL EFFECT OF FRUIT EXTRACT OF EMBLICA OFFICINALIS ON TUBULAR DIAMETER OF SEMINIFEROUS TUBULES OF TESTIS ON ENDOSULFAN INDUCED TESTICULAR TOXICITY OF MALE SWISS ALBINO MICE

Kailash Kumar*

ABSTRACT

The intence use of pesticides as modern chemicals has created a lot of health problem in human including cancer, liver toxicity, male sterility etc, by its persistence nature. Endosulfan, a pesticide of organochlorene group, is being extensively used as an insecticide for different crops. It accumulates in human body through food chain and cause sterility in human beings. It also causes a wide range of harmful health effect in human including cancer, injury in nervous system, lung damage, reproductive dysfunction and sterility. Under present investigation effect of endosulfan on testes of male swiss albino mice was analysed to predict its toxigenic potential and correlated with the histopathology of testis. In my present investigation, endosulfan was administered orally to male swiss albino mice group for 4-weeks @ 3.0 mg/kg b.w. per day for 4 weeks. After that each group of mice were sacrificed and their testis tissue were fixed for light microscopic study. The histopathological study of normal control mice showed tubular diameter of seminiferous tubules varied from 3.12 ± 0.25 to 3.28 ± 0.4 μm, after exposure of Endosulfan To the mice showed the value decreses from 3.02 ± 0.4 to 2.48 ± 0.37 μm. After the administration of Emblica officinalis to the endosulfan treated mice its value increases from 2.89 ± 0.38 to 2.97 ± 0.25 μm. Further it was also found that the number of interstitial cells and spermatogonia decreases after Endosulfan treatment, but after the administration of Emblica officinalis on endosulfan treated mice shows regeneration of spermatogonia and interstitial cells.

Keywords: Male mice, Endosulfan, Testes, Emblica officinalis.