CHROMATOGRAPHIC METHOD FOR DEVELOPMENT AND VALIDATION OF CINNARIZINE USING ICH GUIDELINES

Waghmare Sonali Arun* and Phatangare Nitin Gangadhar

ABSTRACT

In the present research work, a successful attempt was made for determination of Cinnarizine in Bulk form by High performance liquid chromatography. The method was developed by experimentation, based on literature survey. The simplicity, rapidity, reproducibility and economy of the proposed method completely fulfil the objective of this research work. The HPLC method was developed and validated for estimation of Cinnarizine. The mobile phase was consisting of Methanol: water (87:13) pH3. Detection was done at 255 nm. The method was found to be simple, linear, rapid, accurate, precise, reproducible and robust. The % RSD was found within limit. The result showed that proposed method was suitable for the accurate, precise and rapid determination of in Cinnarizine its bulk form. Before making experimentation with ion-exchange or ion-pair chromatography, ion suppression by pH controls and reverse phase chromatography should be tried for ion forming organic compounds. Ion-pair chromatography should be preferred to Ion Exchange chromatography.

Keywords: Cinnarizine, High performance liquid chromatography, Derivative Spectrophotometry, Q absorbance method, Normal Phase Chromatography.