EVALUATION OF ANTI CANCER ACTIVITY OF NOVEL POLYHERBAL FORMULATION

K. Jayaprakash and M. Priya*

ABSTRACT

Cancer is multi pathological disease that is being considered immortal in today’s global medicine world. Therefore, the present study was aimed to develop and evaluate polyherbal formulation (PHF) for its anticancer activity. PHF was prepared by mixing of equal proportion of extracts of Azadirachta indica (leaves), Trigonella foenum-graecum (seeds), Canthium coromandelicum (leaves) and Barringtonia acutangula (bark). The polyherbal formulation was evaluated for their cytotoxic effect using MTT (3-(4,5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay on cell line of human breast adenocarcinoma (MCF-7). Results revealed that the developed polyherbal formulation, showed significant effect on cancer cells (in vitro) by increasing cytotoxicity without causing toxicities and could render prospective candidate for the therapy of cancer. Further clinical trial will be of use in establishing it as a potential anticancer formulation.

Keywords: Invitro cancer activity, polyherbal formulation, cytotoxicity, MTT assay, MCF-7.