ANTIMICROBIAL ACTIVITY OF LIBYAN SALVIA FRUTICOSA MIL AND MULTI-DRUG RESISTANT BACTERIA

Salwa I. Abdulla-Eltawaty*, Abdelnaser Othman Busba, Mohamed-Elfatih A. Omare, Aisha Z. Almagboul, Sanaa O. Yagoub, Asma Alramli, Asim Ahmed and Muhammed Abdalbagi-Dafalla

ABSTRACT

This study was aimed to evaluate the antimicrobial activity and determine the minimum inhibition concentration (MIC) of the Salvia fruticosa extracts against standard microbe strains and clinical bacterial isolates. Methods: Soxhlet apparatus and rotary evaporator with maceration and Freeze drying were used for extraction and evaporation of the organic and aqueous extracts, respectively. Standard methods were used to evaluate the antimicrobial activity and minimum inhibition concentration. Results: The highest activity of extracts against standard strains was shown against the B.subtilis followed by C.lbicans, S.aureus, Ps.aeruginosa and E.coli, with range of inhibition zones of 21 ± 0.7 to 15 ± 0.01, respectively inhibition zones of 21 ± 0.7 to 15 ± 0.01. Among the tested clinical isolates, the methanol bark extract showed good growth inhibition zones ranged from 11.5mm ± 1.6 to17.5mm ± 4 against Gram negative bacteria; Acinetobacter baumanii, Enterobacter cloacae, Escherichia coli, Klebsiella pneumonia, Proteus mirabilis and Pseudomonas aeruginosa and 19mm ± 4.54 against MRSA compared with most of tested antibiotic references, except of Ciprofloxacin 30μg which showed weak activity against A.baumannii, but showed higher activity against other tested clinical isolates. The bark extract showed same activity of Gentamicin10μg against Ps.aeruginosa and MRSA and showed closed performance to that of Ceftazidime 30μg against E.coli. Compared with Augmentin 30μg and Ceftriaxone 30μg, the methanol bark extract Salvia extract was the only effective one against all tested clinical bacteria. Conclusion: This study concluded that Salvia fruticosa extracts have good antibacterial and antifungal activities and suggests that the methanol bark extract have different behaves by which can fights the bacteria. In addition this study gives the assumption that Salvia fruticosa could be considered as a key solution against multidrug resistant pathogenic bacteria.

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