ASSOCIATION BETWEEN HER-2/NEU IHC AND P53, ESTROGEN, PROGESTERONE RECEPTOR (ER, PR) AND KI67 BIOMARKERS

Osman Mohammed Elmahi, Elwaleed Mohamed Elamin, Lamya Ahmed Mohamed El Hassan, Ahmed Mohamed El-Hassan, Hisham Ali Waggiallah*

ABSTRACT

Human epidermal growth factor receptor 2 (Her-2/neu) has been evaluated in endometrial cancer. Aim: This study was conducted to evaluate Association between HER-2/neu IHC and p53, oesrogen, progesterone receptor (ER, PR) and Ki67 biomarkers among Sudanese women. Methods: This was a descriptive cross sectional retrospective cohort study conducted in Khartoum state, Sudan. Eighty one samples of Archival formalin fixed paraffin embedded blocks referred to the histopathology lab in the period from 2007 to 2013 were used in this study. Tissue microarray (TMA) technique was employed in which paraffin blocks were prepared before subjected to different immunostains. Results: This study demonstrated that there is 12(16.3%) of over expression and amplification of Her-2/neu oncoprotein in endometrial carcinoma. No association found between HER-2/neu receptor and other negative prognostic factors of the endometrial cancer. 53 cases (71.6%) showed increased proliferation of the Ki-67 marker. These results are acceptable since it is well known that increased proliferation is responsible for tumor growth. In addition this may explain the significant correlation between Her-2/neu over expression and higher level of Ki-67 since P-value, (0.040). Conclusion: We concluded that there was moderate association between Her2 /neu and Ki-67, whereas no other significant association detected between her 2/ neu and the other biomarker.

Keywords: HER-2/neu, p53, estrogen, progesterone receptor, Ki67