EFFICACY OF MUPIROCIN WITH CHITOSAN AND COLLAGEN AS COMPARED TO MUPIROCIN ONLY ON EXPERIMENTALLY INDUCED INCISION WOUND HEALING IN WISTAR RATS

Mohammed Faraz Khan, Trisha Paloma Rebello, Laxminarayana K. Bairy*, Suresh Kumar S., Vijaya Paul Samuel and Sareesh Naduvil Narayanan

ABSTRACT

Introduction: Wound healing is a process involving hemostasis, inflammation, proliferation and remodeling. Any insult in the process delays the wound healing. The antibiotics have a role in preventing infections and thereby facilitate process of wound healing. Mupirocin is used for Staphylococcus aureus, Streptococcus pyogenes or methicillin-resistant Staphylococcus aureus (MRSA) skin infections. On the other hand, chitosan and collagen are shown to facilitate wound healing. Thus, this study intends to compare mupirocin application versus mupirocin with chitosan and collagen application on incised wounds in Wistar rats. Methods: Fifteen male Wistar rats were divided into three groups (5 rats per group) namely control, mupirocin and mupirocin+chitosan+collagen groups. After overnight fasting, the incision wounds with interrupted sutures were created on dorsum of animals. The animals in group 2 and 3 received topically mupirocin and mupirocin plus chitosan and collagen respectively daily for ten days. The control group received no topical ointment. The sutures were removed on day 7 and wound breaking strength was measured on day 10. Results: The breaking strength of both mupirocin and mupirocin+chitosan+collagen groups were statistically higher than control group. Although the chitosan and collagen addition had increased the breaking strength, there was no statistical difference between mupirocin and mupirocin+chitosan+collagen groups. Conclusion: Mupirocin and mupirocin+chitosan+collagen groups show better healing in rat incision wound model when compared to control group.

Keywords: Wound healing, mupirocin, chitosan, collagen.