SIMULTANEOUS ESTIMATION OF AMBROXOL HYDROCHLORIDE AND LEVOFLOXACIN HEMIHYDRATE USING ABSORBANCE RATIO METHOD

*Bodhankar V. R., Thoke S. T., Kouthekar V. R., Deshmukh S. M. and Jadhao U. T.

ABSTRACT

A simple, specific, accurate, precise and economical spectrophotometric method has been developed for the estimation of Levofloxacin (LVF) and Ambroxol (AMB) in tablet dosage forms. Absorbance ratio method was used. From the overlay spectra of ABH and LFH in distilled water at concentration of 10 mg /mL each, two wavelengths 244.6 nm (λ max of ABH) and 218.6 nm (isoabsorptive point) were selected for estimation of both drugs. ABH and LFH solution individually follows the Beer-Lambert’s law over concentration range 3 - 7 µg/mL and 5 – 25 µg/mL respectively. Different analytical parameters such as linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ), recovery were determined as per ICH guidelines. The recovery values between prescribed limit of 99-100% free from interference of excipients present in formulation. Hence, the developed method can be used quality control analysis for routine.

Keywords: Levofloxacin hemihydrate, Ambroxol hydrochloride, UV spectrophotometric method, Absorbance ratio method, etc.