CAROB POD-A NOVEL SUBSTRATE FOR FRUCTOSE SYRUP AND ETHANOL PRODUCTION USING FRUCTOSE NEGATIVE MUTANTS BY SUBMERGED FERMENTATION

*Ramakrishna D., and **Lingappa K.

ABSTRACT

Use of Carob pods for fructose syrup and ethanol production with two mutant strains Zymomonas mobilis and Saccharomyces cerevisiae were investigated. the carob pod was found to be a better substrate than sugarcane waste, pineapple waste, Banana waste and yielded maximum amount of fructose syrup and ethanol with the mutant strains of KLR5 and KLR3after 120 hrs of fermentation they yielded fructose 400g/lt and ethanol2.8% and Saccharomyces cerevisiae KLR3 yielded 380g/lt and ethanol2.4%. As such carob pod satisfies many parameters of suitability, hence it has been preferred & selected as the suitable substrate.

Keywords: Carob pod, sugarcane Fructose syrup, Ethanol, Zymomonas mobilis, Sacchromyces cerevisiae, submerged fermentation.