DEVELOPMENT AND VALIDATION OF UV SPECTROSCOPIC METHOD FOR THE ESTIMATION OF TICAGRELOR IN TABLET DOSAGE FORM

Meena Iyer*, Sowmya H. G. and Jose Gnana Babu C.

ABSTRACT

Simple, precise and accurate zero order derivative spectroscopic method has been developed and validated for the estimation of Ticagrelor in bulk and Pharmaceutical dosage form. The drug shows maximum absorption (λ max) at 253nm in Acetonitrile solution and obeys Beer’s law in the concentration range of 5-30 μg/ml. The linearity study carried and regression coefficient was found to be 0.9997 and it has showed good linearity, precision during this concentration range. The % recovery was found to be 99.62-99.99. The LOD and LOQ were found to be 0.073 and 0.2209μg/ml. The % relative standard deviation were found less than 2. According to ICH guidelines the method has been validated for linearity, precision, accuracy, robustness, ruggedness, LOD and LOQ. The developed and validated method can be successfully applied for reliable quantification of Ticagrelor in bulk form and pharmaceutical dosage form.

Keywords: Ticagrelor, Zero order derivative spectroscopy, validation, pharmaceutical formulations.