STUDIES ON THE HEPATO AND RENO PROTECTIVE EFFECT OF ALCOHOLIC SEED EXTRACT OF CAESALPINIA BONDUCELLA ON ALLOXAN INDUCED DIABETIC MALE ALBINO WISTAR RATS

Vedavyasa Sagar, R. Nazeer Ahamed* and Subhas C. Bijjaragi

ABSTRACT

Objective: To evaluate the efficacy of alcoholic seed extract of Caesalpinia bonducella (ASECB) in hepato and reno protection in alloxan induced diabetic male albino rats. Methods: The rats were divided into six groups, Normal-Control, Diabetic-Control, Normal+200mg ASECB, Normal+400mg ASECB, Diabetic+200mg ASECB and Diabetic+400mg ASECB. Rats were made diabetic by intraperitoneal injection of alloxan. Oral administration of different doses of ASECB to normal and diabetic rats was conducted for a period of 30 days. Histopathology of liver and kidney and biochemical estimation of urea, uric acid and creatinine was studied. Results: Lobular architecture of liver of control and control-treated rats was normal and undisturbed. In diabetic rats, overall lobular architecture was disturbed. Central veins appear congested, sinusoidal space dilated, hepatic chords disrupted and hepatocytes overlapped and degenerated. In diabetic-treated groups histological disturbances were improved and restored to normalcy. The kidney histology was normal in control and in control-treated rats. In diabetic-control group, the kidney section showed pathological changes in malphigian corpuscles and in urinary tubules. In diabetic-treated rats, the renal corpuscle, tubules and other segments were well maintained and returned to normalcy. The diabetic control group showed significant (P<0.05) increase in renal markers, while the diabetic rats treated with ASECB showed a significant (P<0.05) fall in levels of renal markers. Higher dose of 400 mg was more effective than 200 mg. Conclusion: ASECB has improved the liver and kidney functions by ameliorating histopathological lesions and degenerative effects of alloxan associated with diabetic state. Thus, ASECB serves as a good oral hepato and reno protective agent.

Keywords: Caesalpinia bonducella; Albino rats; Diabetes mellitus; Liver; Kidney.