HORMONAL INFLUENCE ON PHOSPHOLIPASE D AND TRIGLYCERIDE LEVELS IN THE LIVER; INTERACTION WITH PHOSPHATIDIC ACID PHOSPHOHYDROLASE ENZYME

Manoj G Tyagi*, Aniket Kumar, Anand R and S Vilvan

ABSTRACT

Several studies suggest that insulin resistance is associated with alterations in the lipid levels particularly with the triglycerides. This study was conducted to ascertain the effects of pancreatic hormone insulin and adrenergic neurotransmitter norepinephrine (NE) on the phospholipase D (PLD) enzyme activity and triglycerides levels in goat liver. Goat Liver homogenates were subjected to treatment with insulin and norepinephrine and the PLD activity was ascertained. Pretreatment with phosphatidic acid phosphohydrolase inhibitor, Propranolol potentiated the effects of insulin and NE on PLD by 16.28% and 21.32 % respectively. On the other hand PLD inhibitor 2, 3 Bisphosphoglycerate (2,3 BPG) attenuated the effects of insulin and NE on PLD activity. A Similar trend was seen with the 2,3 BPG treatment on triglyceride levels estimated in the goat liver. It can be suggested from our results that phospholipase D enzyme mediated pathway may be partially responsible for the synthesis and regulation of triglyceride levels in the goat liver.

Keywords: Phospholipase D, Triglycerides, Insulin, Norepinephrine, Liver, Propranolol.