PREPARATION OF BSANPS BY USING MODIFIED EMULSO-DESOLVATION METHOD

*Kirti Rani

ABSTRACT

Bovine serum albumin nanoparticles (BSANPs) are considered safe and biocompatible biocarrier systems which are used for effective tragetted delivery carriers. BSANPs are also have standard low-cost and safe nanobiomaterials for improved drug loading capacity for loaded bioactive molecules with high site specificity and low cytotoxicity. Size controlling of BSANPs by using improved and modified methods is noted to be a crucial step that make BSANPs more potent nonviral nanovehicles to get controlled and sustained targeted drug delivery when these are proposed to be bind with other desired components by using Nanotechnology Albumin Binding Technology (nab technology). In present work, BSANPs were prepared by modified emulso-desolvation process using coconut oil and toluene to get most controllable particle size at nanoscale. DLS (Dynamic Light Scatting) was used to characterize the size distribution of the prepared BSANPs. The prepared BSANPs were found to attained size range from 8 nm to 10 nm with exhibited diameter up to 269.2 nm and width of 158.4 nm which are observed to attained good narrow size distribution at nanoscale considerations. Hence, this proposed emulso-desolvation method can be a safe and low-cost technology to prepare fine sized BSANPs and diameter which may prove more potent drug and gene delivery carriers.

Keywords: Bovine serum albumin nanoparticles; BSANPs; Emulso-Desolvation; Coconut oil; Dynamic Light Scattering (DLS).