STUDY COMPARATIVE ANTI-MICROBIAL ACTIVITY OF METHANOLIC EXTRACT OF FLOWERS OF CALOTROPIS GIGANTEA AND CALOTROPIS PROCERA

P. B. Kadam*, D. S. Kachare and K. T. Sul

ABSTRACT

Ayurveda is the oldest medicine system and includes numerous medicinal plants. Medicinal plants are massive source of biologically active compound and offering broad range of activity. The present study was aimed to evaluate and compare antimicrobial activity of methanolic extract of flowers of Calotropis gigantea and Calotropis procera which is also known as Rui. Its antimicrobial activity was performed by using gram negative bacteria Escherichia coli; gram positive bacteria Bacillus subtilis and fungi Aspergillus niger by cup plate method. Streptomycin and Itraconazole was used as a standard and to compare the antimicrobial activity of plants on pathogens. The antimicrobial activity was measured by different concentrations of methanolic extract of flowers of Calotropis gigantea and Calotropis procera with its minimum dose (20μg/ml) and maximum dose (100μg/ml) and Streptomycin (100μg/ml) and Itraconazole (100μg/ml). Antimicrobial activity was evaluated by measuring the zone of inhibition in millimeters. It can be concluded that methanolic extract of Calotropis gigantea and Calotropis procera was found to have antimicrobial activity against gram positive Bacillus subtilis; gram negative E. coli and fungi Aspergillus niger. It also indicated that methanolic extract of pants are potentially good for antibacterial resistant bacteria therapy.

Keywords: Antimicrobial activity, Soxhlet extraction, Ultrasonication, cup plate method.