EFFECT OF CRINUM JAGUS EXTRACTS ON LIPIDS PARAMETERS AND NON ALCOHOLIC HEPATIC STEATOSIS IN MACAPOS 1 INDUCED DIABETES RATS

Clémence Mvongo*, Adamou Mfopa, René Kamgang and Jean-Louis Essame Oyono

ABSTRACT

Diabetes is associated with metabolic disorder, including alterations of lipid profile and fat tissue accumulation, which causes among other pathologies, non-alcoholic fatty liver disease. The aim of this work was to investigate the effect of Crinum jagus extracts on lipids parameters and non-alcoholic hepatic steatosis in MACAPOS 1 induced diabetes rats. To induce diabetes, wistar rats (6-8 weeks old) were fed with high sugar diet (HSD) associated, one month after the beginning of the HSD, with dexamethasone (DXM) injection (25 μg/kg once after 2 days during 3 weeks). During 50 days, diabetic rats once daily orally received Metformine (38 mg/kg b.w.), hydroethanolic (75, 150 mg/kg b.w.) and aqueous (150 mg/kg b.w.) extracts. After the induction, diabetic rats presented, overweight, insulin resistance, polydipsia, dyslipidemia associated with increased visceral fat, liver damage lipid peroxydation associated with decrease in antioxidant defense. Crinum jagus extracts remarkably improved the insulin sensitivity of peripheral tissues, reduced body weight gain, seric total cholesterol, triglycerids and increased the seric level of HDL-cholesterol. These effects led to LDL cholesterol and the atherogenic index reduction. The improvement of the lipid profile was associated with the reduction of visceral fat. The plant extracts also significantly (P ˂ 0.01) reduced the amount of liver TG, the activity of seric transaminases, lipid peroxydation markers (plasma, liver, aorta) and increased tissue protein levels, SOD and catalase activity. The hydroethanolic extract was in a dose-dependant manner more effective than aqueous extract and metformine. These results showed that C. jagus extracts possess insulin sensitizing and antidyslipidemia activities, act against non-alcoholic hepatic steatosis and improve oxidative status on MACAPOS 1 diabetic rats. The results thus support the use of C. jagus in African folk medicine, mostly in obesity, diabetes treatment and likely its complications.

Keywords: Crinum jagus, diabetic rat MACAPOS 1, lipids parameters, insulin sensitivity, hepatic steatosis.