SYNERGISTIC ANTI-ANXIETY ACTIVITY OF DIFFERENT COMPOSITION OF VOLATILE OIL OF EUCALYPTUS AND NEEM OIL BY LIGHT AND DARK MODEL

Amrita Asthana, Vandana R. Nair and *Kishu Tripathi

ABSTRACT

The research work deals with the screening of synergistic anti-anxiety activity of different composition of Eucalyptus (Eucalyptus globulus) and Neem (Azadirachta indica) oils in Swiss albino Mice. In this study were used 9 group (n=5) of mice. Standard, control and test drugs were given to each animal orally; behaviour testing was performed in animal models after 30 min time spent in dark arm was observed for 5 min duration (300 s). Significant increases in percentage of time spend in dark arm of Light and Dark arms indicate anxiety effect respectively. Significant decrease in above parameters indicates anxiogenic effect. Eucalyptus and Neem oil in 1:1, 1:2, 1:3, 2:1 and 3:1 ratios significantly increased percentage of time spend in dark arm of Light and Dark method as compared to vehicle treated mice in both Light and Dark method behavioural models.

Keywords: Anxiety, Eucalyptus and Neem oil, Light and Dark Model, Diazepam.