PHYTOCHEMICAL INVESTIGATIONS AND PHARMACOLOGICAL EVALUATION OF LAWSONIA INERMIS L. BARK FOR HEPATOPROTECTIVE ACTIVITY

Uma Bharti*, Shaily Mishra and Dr. Shamim Ahmad

ABSTRACT

Bark of Lawsonia inermis L. is an evergreen tree, belonging to family Sapotaceae, cultivated in gardens as an ornamental tree. It is traditionally used as cardiotonic, anthelmintic, in the treatment of leprosy and various ailments. The present study includes phytochemical and pharmacological evaluation of bark. Here the powdered bark was subjected to successive soxhlet extraction using different solvents of increasing polarity. The successive methanol and aqueous extracts were subjected for In-vivo hepatoprotective evaluation. The methanolic extract was found more effective. So it was further subjected for preliminary phytochemical and chromatographic examination. Attempt was made to isolate the compounds using isocratic elution technique. The compounds isolated were characterized by UV, FT-IR, 1H-NMR, 13C- NMR, and Mass spectroscopy.

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