HPLC METHOD FOR ESTIMATION OF DEFERASIROX IN PHARMACEUTIICAL FORMULATION AND ENVIRONMENTAL WATER SAMPLES

Nief Rahman Ahmed*, Suhaib Naeyf Lottfie and Yasmin Salim Hassan

ABSTRACT

A simple, precise, rapid, and accurate high performance liquid chromatography method has been developed for the determination of Deferasirox in pure from, pharmaceutical formulations and environmental water samples. Chromatography was carried out on L7 (C8) machereg-nagel column (15cm × 4.6mm), 5 microns, using a mixture of H20: Ethanol (35:65 v/v) adjusted to pH2.5 by H3PO4 as a mobile phase at a flow rate of 1.5 ml.min-1. Detection was performed at 250nm at ambient temperature. The retention time was found 21 minutes. The calibration curve was linear (r= 0.998) over a concentration range from 10 to 200 μg/ml. Limit of detection (LOD) and limit of quantitation (LOQ) were found 0.2377μg/ml and0.7548 μg/ml respectively. The method was validated for its linearity, precision and accuracy. The proposed method was successfully applied for the determination of Deferasirox in pure form, pharmaceutical formulations and in environmental water samples.

Keywords: HPLC, Deferasirox, Pharmaceutical Formulations, Environmental Water samples.