A REVIEW ON RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF AZLINIDIPINE DRUG IN TABLET DOSAGE FORM

Sarita Sasane*, Bhawar H.S., Dighe A.S. and Kolhe S.D.

ABSTRACT

The stability indicating RP-HPLC method was found to be simple, rapid, sensitive, specific, precise, accurate and cost effective for estimation of azelnidipine in tablet dosage form and bulkdrugs substance. This technique was employed in the present investigation for estimation of Azelnidipine using HPLC with Inertsil ODS-3V, 150 x 4.6 mm, 5μ column, UV/PDA detector at 255 nm wavelength, flow rate was 1 ml/min, injection volume with empower Software was used for the study. The standard and sample solution of azelnidipine were prepared in diluent. the column make injection volume and different pure solvents of varying polarity in different proportions were tried as mobile phase for development of the chromatogram. The mobile phase that was found to be most suitable was Buffer pH 3.2 and Methanol, the wavelength 255 nm were selected for the evaluation of the chromatogram of azelnidipine respectively. The selection of the wavelength was based on the λ max obtained by scanning of standard laboratory mixture in water: methanol. This selected chromatographic condition gave good resolutionand optimum retention time with appropriate theoretical plates, tailing factor.

Keywords: .