EVALUATION AND VALIDATION OF A UPLC METHOD FOR ESTIMATION OF MAGALDRATE AND SIMETHICONE IN MARKETED (NUCOOL) SUSPENSION DOSAGE FORM

Dr. Osman Ahmed*, Meher Afrin, Ashraf Unnisa, Mohammed Akthar Sulthana and Dr. Anas Rasheed

ABSTRACT

In order to accomplish separation under optimal circumstances following a series of experimental trials, it is necessary to summarise the results. A stationary phase such as the Hypersil BDS C18 (100 mm x 2.1 mm, 1.7 m) column was the most appropriate since it generated symmetrical peaks with high resolution and a very excellent sensitivity, as well as a very good resolution and sensitivity. The flow rate was kept constant at 1.6 mL min-1, indicating acceptable resolution. The response of Magaldrate and Simethicone PDA detectors was investigated, and it was discovered that the optimal wavelength for achieving the maximum sensitivity was 250 nm. Mobile phase was found to be an acceptable mobile phase for separation of Magaldrate and Simethicone when a combination of two solutions Methanol, Water, and Acetonitrile in the ratio of 40:30:30 percent v/v/v" with gradient programming was utilised at 1.6mL/min. The temperature of the column was kept at room temperature.

Keywords: Suspension dosage form, Magaldrate and Simethicone.