COMPARISON OF IMMUNOMODULATORY ACTIVITY OF ANDROGRAPHIS PANICULATA AND TINOSPORA CORDIFOLIA

Rajesh Yadav*, Nita Yadav and Murli Dhar Kharya

ABSTRACT

The work mainly focus on the immunomodulatory potential of methanol extracts of two Indian medicinal plants- Andrographis paniculata (AP) and Tinospora cordifolia(TC). The coarse powder of aerial parts of AP and mature stems of TC (500g each) was subjected separately to successive extraction with 1000ml each of petroleum ether (60-80oC) followed by methanol. The dose of methanol extracts were selected and were administered orally at doses of 200 mg/kg body weight to healthy rats and compared with the control group and standard drug i.e. cyclophosphamide (100mg/kg) respectively by using different animal models like modulation of macrophage function, humoral response in normal and cyclophosphamide induced immunodeficient rats, neutrophil adhesion (NA) test, haemagglutinating antibody (HA) titre, delayed type hypersensitivity (DTH) response in rats. The methanol extract of the aerial parts of A. paniculata exhibited appreciable activity as compared to the methanol extract of mature stems of Tinospora cordifolia. After arriving to a conclusion that Andrographis paniculata exhibited best immunostimulant potential, indicating that it has promising immunomodulatory activity, as proposed from commercial point of view.

Keywords: Methanol extract, Andrographis paniculata, Tinospora cordifolia Haemagglutination, Humoral and cellular responses, Immunostimulant.