THE EFFECT OF D-RIBOSE-L-CYSTEINE ON INDOMETHACIN INDUCED OXIDATIVE STRESS AND GASTRIC ULCERATION IN ADULT MALE ALBINO WISTAR RATS

Rosemary N. Orjiako*, Edwin Nwobodo, Peter Okonudo, Chukwudi Onyeka Okonkwo, Uzozie Chikere Ofoego, Chukwunenye Chibuike Ubah

ABSTRACT

Gastric ulceration is the most predominant gastrointestinal syndrome ever recognized. The major etiological factors in the pathogenesis of peptic ulcer disease which is H.Pylori as well as increasing use of NSAIDS lead to increased production of ROS by immune and epithelial cells and by activated neutrophils respectively, resulting in oxidative stress. However, studies have shown that Riboceine is a potent anti-oxidant. The aim of the study was to investigate the effect of D-Ribose-L-Cysteine on indomethacin induced Oxidative stress and gastric ulceration in adult male albino wistar rats. 24 adult male albino wistar rats were randomly divided into four groups of six rats (n=6); normal control group (Grp. 1-received normal saline only), ulcer control group (Grp. 2- received normal saline with indomethacin), treatment group 3 (pre-treated with riboceine for three days), treatment group 4 (pre-treated with riboceine for seven days). Riboceine was administered at 2mg/kg to the animals in the treatment groups. Riboceine administration significantly reduced stomach weight (P<0.05). The increased ulcer index due to indomethacin administration was significantly ihibited following Riboceine pre-treatment for three days and seven days (P<0.05). Riboceine administration for three and seven days significantly reduced malondialdehyde level (P<0.05). Riboceine administration for seven days significantly increased catalase activity (P<0.05). Administration of Riboceine for seven days also significantly increased SOD (P<0.05). In conclusion, this study showed that riboceine can be used in the prevention of gastric ulceration due to its gastroprotective and antioxidant potentials.

Keywords: Riboceine, indomethacin, oxidative stress, gastric ulceration, antiulcerogenic, antioxidant.