EVALUATION OF ANALGESIC AND ANTI OXIDANT ACTIVITIES OF SCHIFF BASES

Shan P. Mohammed*, Namsheer B, Dr.Jyoti harindran, Beena Thomas and Mithun K. Sebastian

ABSTRACT

Schiff bases which contain an azomethine group attract much interest in synthetic chemistry. Schiff bases are used as substrates in the preparations of a number of industrial and biologically active compounds via closure, cycloaddition and replacement reactions. Moreover, Schiff bases are also known to have biological activities such as antibacterial, antifungal, antitumor, antioxidant and analgesic activities. INH Schiff bases have better anti-tubercular activity than parent drug isoniazid. In the present study, three different Schiff bases of INH were prepared and the analgesic and anti-oxidant activities were investigated. Analgesic activity was evaluated by acetic-acid induced writhing test in which the Diclofenac was used as the standard drug. In vitro antioxidant activity of the INH Schiff base analogues were evaluated using DPPH free radical scavenging method. In acetic acid induced writhing test, the study showed that there was a significant inhibitory effect on writhing by all the compounds. DPPH radical scavenging activity of the three INH Schiff base analogues at different concentrations (100-500μg/ml) was compared with standard antioxidant ascorbic acid (100-500μg/ml). The compounds showed significant increase in free radical scavenging activity in a dose dependent manner.

Keywords: azomethine, diclofenac ,schiff base,DPPH,acetic acid.