NITRIC OXIDE RADICAL SCAVENGING ACTIVITIES IN EFFECTIVE MICROORGANISMS (EM) AND COLLAGEN

Man Kyu Huh* and In Sook Kye

ABSTRACT

This study was to evaluate the nitric oxide (NO) radical scavenging activities extracts from effective microorganisms (EM) with/without collagen extracts. Collagen has a unique triple-helix structure with a repeated amino acid sequence of (Gly-X-Y)n, in which X and Y are typically Pro and Hyp. EM is various blends of common predominantly anaerobic microorganisms consisting of a culture of potentially beneficial microorganisms. Experiments were conducted in anticipation of the EM fermentation solution decomposing polymer collagen into low molecular weight collagen. It was observed that inhibition percentage values go on increasing with enhancements in concentration of research EM and collagen extracts in the assay mixture. NO scavenging activity of the EM-A was evaluated 60.0% on 100% solution without collagen and that of the EM-B was 47.6% at same concentration. Three EM groups with collagen were shown higher radical scavenging effects at all concentration than those of EM groups without collagen. The values of inhibits 50% of the enzyme activity (IC50) for the EM-A, EM-B, and EM-C were 264.6, 303.5, and 358.1 μg/ml, respectively.

Keywords: Collagen, Effective microorganisms (EM), nitric oxide radical scavenging.