A NEW METHOD DEVELOPMENT FOR ETOPHYLLINE AND THEOPHYLLINE SIMULTANEOUS ESTIMATION BY RP-HPLC AND VALIDATION

K. L. Rajita*

ABSTRACT

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Etophylline and Theophylline, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Phenomenex Luna C18 (4.6×150mm, 5μ) column using a mixture of Acetonitrile: Water (10:90% v/v) as the mobile phase at a flow rate of 0.9ml/min, the detection was carried out at 240nm. The retention time of the Etophylline and Theophylline was 1.933, 3.396 ±0.02min respectively. The method produce linear responses in the concentration range of 16.5-82.5mg/ml of Etophylline and 5-25mg/ml of Theophylline. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.

Keywords: Etophylline, Theophylline, RP-HPLC, validation.