A VALIDATED METHOD DEVELOPMENT FOR THE ESTIMATION OF SEMAGLUTIDE IN ITS BULK AND PHARMACEUTICAL DOSAGE FORM BY USING UV-VISIBLE SPECTROPHOTOMETER
K.L. Rajita*
ABSTRACT
A rapid and precise UV-VISIBLE spectrophotometric method has been developed for the estimation of semaglutide in its bulk and pharmaceutical dosage form. Spectrophotometry was carried out on a UV-Visible spectrophotometer (UV-T60-India) in quartz cells using ethanol as suitable solvent, the detection is carried out at 288nm.The pre-determined wavelength of maximum absorption (λmax) was 288nm. The drug obeyed Beer-Lambert’s law over the concentration range 5-30μg/ml. The accuracy retrived by recovery studies was found to be 99.92% for 50% concentration, 99.08% for 100% concentration, 99.95% for 150% concentration. This method can be employed for routine analysis of semaglutide in bulk and pharmaceutical dosage form.
Keywords: Semaglutide, estimation, Ozempic.
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