SERIAL DILUTION & IT’S USE IN HPLC

*Dr. Dhrubo Jyoti Sen, Kushal Nandi and Dr. Dhananjoy Saha

ABSTRACT

Dilution is the process of decreasing the concentration of a solute in a solution, usually simply by mixing with more solvent like adding more water to the solution. To dilute a solution means to add more solvent without the addition of more solute. The resulting solution is thoroughly mixed so as to ensure that all parts of the solution are identical. The same direct relationship applies to gases and vapors diluted in air for example. Although, thorough mixing of gases and vapors may not be as easily accomplished. For example, if there are 10 grams of salt (the solute) dissolved in 1 litre of water (the solvent), this solution has a certain salt concentration (molarity). If one adds 1 litre of water to this solution, the salt concentration is reduced. The diluted solution still contains 10 grams of salt (0.171 moles of NaCl).

Keywords: Dilution Liquid, HPLC, UV- Spectroscopy.