CHARACTERIZATION OF QUAIL IGY PRODUCED AGAINST NEWCASTLE DISEASE VIRUS

Mai F. Nahla, Naglaa M. Hagag and Madiha Salah Ibrahim*

ABSTRACT

The IgY technology can be used for the production of antibodies for immunological analysis, disease diagnosis and control. Newcastle disease (ND) is one of the most important viral diseases affecting birds worldwide and is responsible for serious economic losses to the poultry industry due to high mortality, decreased egg production, and body weight loss. Further, it requires additional approaches together with vaccination to overcome its endemicity. In this study, a total of 50 Japanese quails (Coturnix coturnix japonica) 4-week old were used for the production of IgY against NDV genotype VII. The produced quail IgY was tested for titers and specificity using Hemagglutination-inhibition (HI) and agar gel immune diffusion test (AGID). The produced IgY was tested for purity by SDS-page analysis. HI showed gradual increase in the IgY titers till the end of the experiment. Further, AGID showed positive interaction between the produced IgY and filed samples infected with NDV. This collectively indicates the development of significant titers of purified IgY against NDV genotype VII virus in quail eggs.

Keywords: Newcastle disease virus, Quails, IgY, HI, AGID.